Form of Antibody:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
pk10牛牛Storage/Stability:Store at -20℃/1 year
Immunogen:The antiserum was produced against synthesized phosphopeptide derived from human Akt around the phosphorylation site of serine 473 (Q-F-SP-Y-S).
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
pk10牛牛Specificity/Sensitivity:Akt (phospho-Ser473) antibody detects endogenous levels of Akt only when phosphorylated at serine 473.
Reactivity:Human, Mouse, Rat
Applications:WB: 1:500~1:1000 IHC: 1:50~1:100
pk10牛牛References:Baudhuin LM, et al. (2004) FASEB J Feb; 18(2): 341-3.
pk10牛牛Research Area:Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells